Study on enzyme properties of bacillus subtilis LC-9 multifunction cellulose

2nd International Symposium on Resource Exploration and Environmental Science

生态环境科学

Sui, Ming^1,2 ; Li, Chunming^2 ; Li, Guoying^1,3 ; Zhou, Rongqing^1,3

Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu

610065, China^1

Department of Wine and Food Engineering, Si Chuan Technology and Business College, Si chuan, Du jiang-yan

611800, China^2

National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu

610065, China^3

关键词: Bacillus Subtilis;    Chemical products;    Endoglucanases;    Enzyme properties;    Purification method;    Renewable resource;    Soluble sugars;    Specific activity;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/170/2/022106/pdf
DOI  :  10.1088/1755-1315/170/2/022106

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Cellulose is the most abundant renewable resource in nature and can be used for the production of soluble sugars, biofuels and as raw materials for other important chemical products. Therefore, the development and research of multifunction's enzyme is of great significance for the degradation and utilization of cellulose. In this study, two-step DEAE-Sepharose FF weak anion exchange of fermentation supernatant of Bacillus subtilis LC-9 with different pH buffer as mobile phase was used to obtain the electrophoretic ally pure protein with a molecular weight of about 32 KDa, 25 KDa. The specific activity of filter paper comprehensively characterized by enzyme was 22.68U/mg, the purification fold was 33.35 and the recovery rate was 20.52%. In this study, the purified multifunction's enzyme not only has the activity of endoglucanase, xylanase, filter paper enzyme, exoglucanase and other cellulases, but also has the advantages of short enzyme producing time, simple purification method And other advantages, has a good prospect of industrialization.