Effect of Citrus Unshiu Peel Extract Fermented by Aspergillus niger on Obesity and Hyperglycemia in High­Fat Diet Fed C57BL/6J Mice

Citrus unshiu peel;fermentation;Aspergillus niger;flavonoid;anti­obesity;anti­hyperglycemia;641

생활과학대학 식품영양학과 ;

University:서울대학교 대학원

关键词: Citrus unshiu peel;    fermentation;    Aspergillus niger;    flavonoid;    anti­obesity;    anti­hyperglycemia;    641;   

Others  :  http://s-space.snu.ac.kr/bitstream/10371/133985/1/000000140860.pdf

美国|英语

来源: Seoul National University Open Repository

【 摘 要 】

LFD (low­fat diet), HFD (high­fat diet), CPE (HFD + 1% (w/w) CPE), FCPE 0.3% (HFD + 0.3% (w/w) FCPE) and FCPE 1% (HFD + 1% (w/w) FCPE). FCPE 0.3% group and FCPE 1% group had significantly lower body weight gains (14.1% and 13.2% less respectively, p＜0.05) and epididymal fat weight (28.8% and 23.5% less respectively, p＜0.05). The fasting blood glucose levels were significantly lower in the FCPE groups in comparison to the HFD group (p＜0.05). In intraperitoneal glucose tolerance test, AUC level was significantly lower in the FCPE 0.3% group compared to the HFD group (p＜0.05). In morphology of liver, lipid droplet accumulation was less pronounced in the FCPE0.3% group and FCPE 1% group. Adipocyte size of epididymal fat was lower in the FCPE groups. Furthermore, the levels of total cholesterol and LDL cholesterol in plasma were significantly lower in the FCPE groups (p＜0.05). Expression of genes related to hepatic glucose homeostasis (Gk, G6pase, Pepck), hepatic glucose transporter (Glut2), hepatic lipogenesis and fatty acid oxidation (Srebp1c, Acc, Fas, Cpt1) as well as hepatic cholesterol homeostasis (Srebp2, Hmgr, Pcsk9, Acat2, Cyp7a1, Ldlr) were determined by real­time PCR. The mRNA levels of Gk, Glut2 and CPT1 were significantly higher in the FCPE groups compared with the HFD group (p＜0.05). In addition, FCPE groups had significantly lower Srebp1c, Srebp2, Fas, Acc, Hmgr and Pcsk9 mRNA expression (p＜0.05). In conclusion, FCPE supplementation may function to improve hepatic lipid and cholesterol metabolism as well as hepatic glucose metabolism in high­fat fed mice. These results suggest that FCPE, which contains naringenin, is a fermented food material that has promising effect in amelioration of obesity and obesity-­induced hyperglycemia.