Electro-gene transfer to skin using a noninvasive multielectrode array

JOURNAL OF CONTROLLED RELEASE

卷:151

Article

Guo, Siqi¹  Donate, Amy^1,2  Basu, Gaurav¹  Lundberg, Cathryn¹  Heller, Loree¹  Heller, Richard^1,2 

[1] Old Dominion Univ, Frank Reidy Res Ctr Bioelect, Norfolk, VA 23508 USA

[2] Univ S Florida, Dept Mol Med, Tampa, FL 33612 USA

关键词: Electroporation;    Gene delivery;    Guinea pig;    Skin;    Multielectrode array;   

DOI  :  10.1016/j.jconrel.2011.01.014

来源: Elsevier

【 摘 要 】

Because of its large surface area and easy access for both delivery and monitoring, the skin is an attractive target for gene therapy for cutaneous diseases, vaccinations and several metabolic disorders. The critical factors for DNA delivery to the skin by electroporation (EP) are effective expression levels and minimal or no tissue damage. Here, we evaluated the non-invasive multielectrode array (MEA) for gene electrotransfer. For these studies we utilized a guinea pig model, which has been shown to have a similar thickness and structure to human skin. Our results demonstrate significantly increased gene expression 2 to 3 logs above injection of plasmid DNA alone over 15 days. Furthermore, gene expression could be enhanced by increasing the size of the treatment area. Transgene-expressing cells were observed exclusively in the epidermal layer of the skin. In contrast to caliper or plate electrodes, skin EP with the MEA greatly reduced muscle twitching and resulted in minimal and completely recoverable skin damage. These results suggest that EP with MEA can be an efficient and non-invasive skin delivery method with less adverse side effects than other EP delivery systems and promising clinical applications. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free