In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

Biological Procedures Online

Pernille Hojman¹  Jens Eriksen²  Julie Gehl² 

[1] Centre of Inflammation and Metabolism, Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark

[2] Department of Oncology, 54B1, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark

关键词: Skeletal muscle;    Katushka;    Whole-body imaging;    Gene delivery;    Electroporation;   

Others  :  797175
DOI  :  10.1007/s12575-009-9005-0

 received in 2009-02-25, accepted in 2009-03-05,  发布年份 2009

【 摘 要 】

DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain fluorescence in vivo imaging. Highly efficient transgenic expression was observed after DNA electrotransfer with 100-fold increase in fluorescent intensity. The fluorescent signal peaked 1 week after transfection and returned to background level within 4 weeks. Katushka expression was not as stable as GFP expression, which was detectable for 8 weeks. Depth and 3D analysis proved that the expression was located in the target muscle. In vivo bio-imaging using the novel Katushka fluorescent protein enables excellent evaluation of the transfection efficacy, and spatial distribution, but lacks long-term stability.

【 授权许可】

   
2009 Hojman et al.

【 图 表 】

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