Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:131

Article

Hulse, Kathryn E.¹  Norton, James E.¹  Suh, Lydia¹  Zhong, Qiu²  Mahdavinia, Mahboobeh¹  Simon, Patrick¹  Kern, Robert C.²  Conley, David B.²  Chandra, Rakesh K.²  Tan, Bruce K.²  Peters, Anju T.¹  Grammer, Leslie C., III¹  Harris, Kathleen E.¹  Carter, Roderick G.¹  Kato, Atsushi¹  Schleimer, Robert P.¹ 

[1] Northwestern Univ, Feinberg Sch Med, Div Allergy Immunol, Dept Med, Chicago, IL 60611 USA

[2] Northwestern Univ, Feinberg Sch Med, Dept Otolaryngol, Chicago, IL 60611 USA

关键词: Chronic rhinosinusitis;    B cells;    plasma cells;    antibodies;    EBV-induced protein 2 (EBI2);    chronic inflammation;   

DOI  :  10.1016/j.jaci.2013.01.043

来源: Elsevier

【 摘 要 】

Background: Despite the high prevalence and morbidity of chronic rhinosinusitis (CRS), little is known about the mechanisms that underlie its pathogenesis. Recent studies have suggested that B cells might play an important role in CRS. Objective: We sought to thoroughly characterize B lineage cells within sinus tissues of patients with CRS and healthy control subjects and to determine whether levels of EBV-induced protein 2, which is known to play an important role in the development of B-cell responses, were increased in patients with CRS. Methods: Cells isolated from sinus tissues of patients with CRS and healthy control subjects were characterized by means of flow cytometry and immunohistochemistry. Local production of antibodies was measured in tissue extracts, nasal lavage fluid, and sera by using multiplex bead arrays and ELISA. Quantitative RT-PCR, ELISA, and Western blotting were used to assess gene and protein expression from tissue extracts. Results: Nasal polyps (NPs) from patients with CRS had increased levels of both B cells and plasma cells compared with uncinate tissue from healthy control subjects (P <.05). NPs also contained significantly increased levels of several antibody isotypes compared with normal uncinate tissue (P <.05), but no differences in circulating antibody levels were found. Interestingly, levels of EBV-induced protein 2 were also increased in NPs (P <.05) and were positively correlated with expression of plasma cell markers (CD138 and B lymphocyteinduced maturation protein) in sinus tissue. Conclusion: B cells and plasma cells are enriched in NPs, actively produce antibodies locally, and might contribute to chronic inflammation in patients with CRS. Elucidating the mechanisms that underlie this excessive local B-cell response might provide novel insights for the development of improved therapeutic strategies. (J Allergy Clin Immunol 2013; 131:1075-83.)

【 授权许可】

Free