Label-acquired magnetorotation for biosensing: An asynchronous rotation assay

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:323

Article

Hecht, Ariel^1,2  Kinnunen, Paivo^2,3  McNaughton, Brandon^1,2,3  Kopelman, Raoul^1,2,3 

[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Appl Phys Program, Ann Arbor, MI 48109 USA

关键词: Biosensing;    Diagnostics;    Magnetic beads;    Magnetic rotation;    Biotin;    Streptavidin;    Sandwich Immunoassay;   

DOI  :  10.1016/j.jmmm.2010.09.014

来源: Elsevier

【 摘 要 】

This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude. (C) 2010 Elsevier B.V. Allrightsreserved.

【 授权许可】

Free