Macrophage and tumor cell responses to repetitive pulsed X-ray radiation

5th International Congress on Energy Fluxes and Radiation Effects 2016

Buldakov, M.A.^1,2 ; Tretyakova, M.S.^2 ; Ryabov, V.B.^3 ; Klimov, I.A.^1 ; Kutenkov, O.P.^4 ; Kzhyshkowska, J.^2,3 ; Bol'Shakov, M.A.^2,4 ; Rostov, V.V.^4 ; Cherdyntseva, N.V.^1,2

Department of Molecular Oncology and Immunology, Cancer Research Institute, Tomsk NRMC, Kooperativnii str. 5, Tomsk

634050, Russia^1

Laboratory for Translational Cellular and Molecular Biomedicine, Tomsk State University, Pr. Lenina, 36, Tomsk

634050, Russia^2

Department of Innate Immunity and Tolerance, Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, Mannheim

68167, Germany^3

Physical Electronic Department, Institute of High-current Electronic SB RAS, 2/3 Akademichesky Avenue, Tomsk

634055, Russia^4

关键词: Antitumor effect;    Cytokine secretions;    Lewis lung carcinomata;    Lung metastasis;    Macrophage phenotypes;    Magnetic beads;    Pulse repetition frequencies;    X ray radiation;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/830/1/012045/pdf
DOI  :  10.1088/1742-6596/830/1/012045

来源: IOP

【 摘 要 】

To study a response of tumor cells and macrophages to the repetitive pulsed low-dose X-ray radiation. Methods. Tumor growth and lung metastasis of mice with an injected Lewis lung carcinoma were analysed, using C57Bl6. Monocytes were isolated from a human blood, using CD14+ magnetic beads. IL6, IL1-betta, and TNF-alpha were determined by ELISA. For macrophage phenotyping, a confocal microscopy was applied. "Sinus-150" was used for the generation of pulsed X-ray radiation (the absorbed dose was below 0.1 Gy, the pulse repetition frequency was 10 pulse/sec). The irradiation of mice by 0.1 Gy pulsed X-rays significantly inhibited the growth of primary tumor and reduced the number of metastatic colonies in the lung. Furthermore, the changes in macrophage phenotype and cytokine secretion were observed after repetitive pulsed X-ray radiation. Conclusion. Macrophages and tumor cells had a different response to a low-dose pulsed X-ray radiation. An activation of the immune system through changes of a macrophage phenotype can result in a significant antitumor effect of the low-dose repetitive pulsed X-ray radiation.