Enzyme Functionalized AuNPs and Glucometer-based Protein Detection

5th Annual International Conference on Material Science and Engineering

Dai, Tao^1 ; Fang, Jie^1 ; Yu, Wen^1 ; Xie, Guoming^1

Key Laboratory of Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, China^1

关键词: Double stranded DNA;    Functionalized;    Glucose concentration;    Magnetic beads;    Protein detection;    Protein recognition;    Selective detection;    Target proteins;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/275/1/012010/pdf
DOI  :  10.1088/1757-899X/275/1/012010

来源: IOP

【 摘 要 】

We here developed a novel method for protein detection by using protein aptamer-functionalized magnetic beads for protein recognition and invertase-functionalized AuNPs catalyze sucrose generate glucose that can be detected by a glucometer. First, the invertase and DNA probe P2 are immobilized onto the gold nanoparticles (I.P2@AuNPs). Next protein aptamer P1 are immobilized onto the streptavidin-coated Magnetic beads (P1@MB). P1 and P2 can complementary to form double-stranded DNA. When target protein presence, P1 combine with target and release I/P2@AuNPs. Then magnetic separation, take supernatant fluid and add sucrose after a period of reaction, detection of glucose concentration by glucometer, thus achieve the sensitive and selective detection of the target protein.