5th Annual International Conference on Material Science and Engineering | |
Enzyme Functionalized AuNPs and Glucometer-based Protein Detection | |
Dai, Tao^1 ; Fang, Jie^1 ; Yu, Wen^1 ; Xie, Guoming^1 | |
Key Laboratory of Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, 400016, China^1 | |
关键词: Double stranded DNA; Functionalized; Glucose concentration; Magnetic beads; Protein detection; Protein recognition; Selective detection; Target proteins; | |
Others : https://iopscience.iop.org/article/10.1088/1757-899X/275/1/012010/pdf DOI : 10.1088/1757-899X/275/1/012010 |
|
来源: IOP | |
【 摘 要 】
We here developed a novel method for protein detection by using protein aptamer-functionalized magnetic beads for protein recognition and invertase-functionalized AuNPs catalyze sucrose generate glucose that can be detected by a glucometer. First, the invertase and DNA probe P2 are immobilized onto the gold nanoparticles (I.P2@AuNPs). Next protein aptamer P1 are immobilized onto the streptavidin-coated Magnetic beads (P1@MB). P1 and P2 can complementary to form double-stranded DNA. When target protein presence, P1 combine with target and release I/P2@AuNPs. Then magnetic separation, take supernatant fluid and add sucrose after a period of reaction, detection of glucose concentration by glucometer, thus achieve the sensitive and selective detection of the target protein.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
Enzyme Functionalized AuNPs and Glucometer-based Protein Detection | 375KB | download |