Malaria Journal | |
Direct transfer of HRPII-magnetic bead complexes to malaria rapid diagnostic tests significantly improves test sensitivity | |
Methodology | |
Nicholas M. Adams1  Thomas F. Scherr1  David W. Wright2  Keersten M. Ricks2  Frederick R. Haselton3  | |
[1] Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA;Department of Chemistry, Vanderbilt University, 37235, Nashville, TN, USA;Department of Chemistry, Vanderbilt University, 37235, Nashville, TN, USA;Department of Biomedical Engineering, Vanderbilt University, 37235, Nashville, TN, USA; | |
关键词: Malaria; Diagnostics; Extraction; Magnetic beads; Biomarker concentration; | |
DOI : 10.1186/s12936-016-1448-6 | |
received in 2016-04-15, accepted in 2016-07-20, 发布年份 2016 | |
来源: Springer | |
【 摘 要 】
BackgroundThe characteristic ease of use, rapid time to result, and low cost of malaria rapid diagnostic tests (RDTs) promote their widespread use at the point-of-care for malaria detection and surveillance. However, in many settings, the success of malaria elimination campaigns depends on point-of-care diagnostics with greater sensitivity than currently available RDTs. To address this need, a sample preparation method was developed to deliver more biomarkers onto a malaria RDT by concentrating the biomarker from blood sample volumes that are too large to be directly applied to a lateral flow strip.MethodsIn this design, Ni–NTA-functionalized magnetic beads captured the Plasmodium falciparum biomarker HRPII from a P. falciparum D6 culture spiked blood sample. This transfer of magnetic beads to the RDT was facilitated by an inexpensive 3D-printed apparatus that aligned the sample tube with the sample deposition pad and a magnet beneath the RDT. Biomarkers were released from the bead surface onto the lateral flow strip using imidazole-spiked running buffer. Kinetics of HRPII binding to the Ni–NTA beads as a function of blood sample volume were explored prior to determining the effect of the proposed method on the limit of detection of Paracheck RDTs.ResultsMore than 80 % of HRPII biomarkers were extracted from blood sample volumes ranging from 25 to 250 µL. The time required to reach 80 % binding ranged from 5 to 60 min, depending on sample volume. Using 250 μL of blood and a 30-min biomarker binding time, the limit of detection of the Paracheck Pf RDT brand was improved by 21-fold, resulting in a limit of detection below 1 parasite/μL.ConclusionsThis approach has the sensitivity and simplicity required to assist in malaria elimination campaigns in settings with limited access to clinical and laboratory resources.
【 授权许可】
CC BY
© The Author(s) 2016
【 预 览 】
Files | Size | Format | View |
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RO202311109568571ZK.pdf | 1384KB | download | |
MediaObjects/40538_2023_474_MOESM9_ESM.xlsx | 13KB | Other | download |
Fig. 2 | 192KB | Image | download |
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Fig. 2
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