Frontiers in Immunology | |
Active tuberculosis patients have high systemic IgG levels and B-cell fingerprinting, characterized by a reduced capacity to produce IFN-γ or IL-10 as a response to M.tb antigens | |
Immunology | |
Everardo Curiel-Quesada1  Juan Carlos Cancino-Diaz2  Araceli Contreras-Rodriguez2  Rogelio Hernández-Pando3  Alexia Urbán-Solano4  Lucero A. Ramón-Luing4  Leslie Chavez-Galan4  Julio Flores-Gonzalez5  | |
[1] Department of Biochemistry, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico;Department of Microbiology, Laboratory of Immunomicrobiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico;Department of Pathology, Section of Experimental Pathology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico;Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico;Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosio Villegas, Mexico City, Mexico;Department of Microbiology, Laboratory of Immunomicrobiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico; | |
关键词: tuberculosis; IFN-g; IL-10; B cells; humoral immunity; flow cytometry; | |
DOI : 10.3389/fimmu.2023.1263458 | |
received in 2023-07-19, accepted in 2023-10-10, 发布年份 2023 | |
来源: Frontiers | |
【 摘 要 】
IntroductionTuberculosis (TB) is a bacterial infection caused by Mycobacterium tuberculosis (M.tb). B cells are the central mediator of the humoral response; they are responsible for producing antibodies in addition to mediating other functions. The role of the cellular response during the TB spectrum by B cells is still controversial.MethodsIn this study, we evaluated the distribution of the circulating B cell subsets in patients with active and latent TB (ATB and LTB, respectively) and how they respond to stimuli of protein or lipid from M.tb.ResultsHere, we show that ATB patients show an immune fingerprinting. However, patients with drug-sensitive- (DS-TB) or drug-resistant- (DR-TB) TB have altered frequencies of circulating B cells. DS-TB and DR-TB display a unique profile characterized by high systemic levels of IFN-γ, IL-10, IgG, IgG/IgM ratio, and total B cells. Moreover, B cells from DR-TB are less efficient in producing IL-10, and both DS-TB and DR-TB produce less IFN-γ in response to M.tb antigens.ConclusionThese results provide new insights into the population dynamics of the cellular immune response by B cells against M.tb and suggest a fingerprinting to characterize the B-cell response on DR-TB.
【 授权许可】
Unknown
Copyright © 2023 Flores-Gonzalez, Urbán-Solano, Ramón-Luing, Cancino-Diaz, Contreras-Rodriguez, Curiel-Quesada, Hernández-Pando and Chavez-Galan
【 预 览 】
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RO202311140330227ZK.pdf | 8755KB | download |