The Effect of Sticopus Hermanii-Hyperbaric Oxygen Therapy to Inflammatory Response of Diabetic Periodontitis

12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry

生物科学;化学

Mulawarmanti, D.^1^2 ; Parisihni, K.^1 ; Widyastuti^3

Department of Oral Biology, Faculty of Dentistry, Hang Tuah University, Surabaya, Indonesia^1

Department of Biochemistry, Faculty of Dentistry, Hang Tuah University, Surabaya, Indonesia^2

Department of Periodontology, Faculty of Dentistry, Hang Tuah University, Surabaya, Indonesia^3

关键词: diabetic-periodontitis;    Hyperbaric oxygen;    Hyperbaric oxygen therapies;    IL-10;    Immunohistochemistry;    Inflammatory response;    Sticophus hermanii;    Topical application;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012060/pdf
DOI  :  10.1088/1755-1315/217/1/012060

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Periodontitis is a major risk factor in the oral cavity in chronic diabetes mellitus. The level of IL-1β as inflammatory cytokine increased in patients with type 2 diabetes and periodontitis, while IL-10 is an anti-inflammatory cytokine, may mediate periodontitis in diabetes. Stichopus hermanii (SH) have been known to have anti-inflammation property. Hyperbaric Oxygen Therapy (HBOT) thas been used as adjuvant therapy in diabetes wound healing. Purpose: To examine the effect of combination SH and HBOT to the expression of inflammatory response of IL-1β and IL-10 on diabetic periodontitis rats. Methods :Thirty male Wistar rats were divided to normal (K-0), diabetic-periodontitis (K-1), and treatment group of SH (K-2), HBOT (K-3), and SH-HBOT (K-4).Experimental diabetic was induced by single dose 65 mg/kg of BW intraperitoneal. The Sticopus hermanii gel 3% by topical application in sulcus gingiva and HBOT 2,4 ATA 3x30 minutes interval 5 minute for 7 days. After 52 days the animals were decapitated and the expression of IL-1β and IL-10 were examined by immunohistochemistry. Data were analyzed with ANOVA and LSD. Results : The expression of IL-1β were raised in K-1 group (10.68±1.50) (p0.05). Treatment with SH in K-2 group resulted in decreased of IL-1β expression (10.00± 1.09) while the expression of IL-10 were raised (8.33 ± 2.60) compare to K-1 (7.25 ± 0.85) (p