Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline

BMC Biology

Research Article

Artem Barski¹  Andrey V. Kartashov¹  Satoshi H. Namekawa²  Ho-Su Sin³  Kazuteru Hasegawa⁴ 

[1] Division of Allergy and Immunology, Division of Human Genetics, Cincinnati Children’s Hospital Medical Center, 45229, Cincinnati, OH, USA;Department of Pediatrics, University of Cincinnati College of Medicine, 49229, Cincinnati, OH, USA;Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, 45229, Cincinnati, OH, USA;Department of Pediatrics, University of Cincinnati College of Medicine, 49229, Cincinnati, OH, USA;Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, 45229, Cincinnati, OH, USA;Department of Pediatrics, University of Cincinnati College of Medicine, 49229, Cincinnati, OH, USA;Present address: Department of Developmental Biology, Department of Genetics, Stanford University School of Medicine, 94305, Stanford, CA, USA;Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children’s Hospital Medical Center, 45229, Cincinnati, OH, USA;Department of Pediatrics, University of Cincinnati College of Medicine, 49229, Cincinnati, OH, USA;Present address: Department of Medicine, Stanford University School of Medicine, 94305, Stanford, CA, USA;

关键词: Germ cells;    Epigenome;    Sex chromosomes;    Meiosis;    Spermatogenesis;   

DOI  :  10.1186/s12915-015-0159-8

 received in 2015-03-25, accepted in 2015-06-18,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe male germline transcriptome changes dramatically during the mitosis-to-meiosis transition to activate late spermatogenesis genes and to transiently suppress genes commonly expressed in somatic lineages and spermatogenesis progenitor cells, termed somatic/progenitor genes.ResultsThese changes reflect epigenetic regulation. Induction of late spermatogenesis genes during spermatogenesis is facilitated by poised chromatin established in the stem cell phases of spermatogonia, whereas silencing of somatic/progenitor genes during meiosis and postmeiosis is associated with formation of bivalent domains which also allows the recovery of the somatic/progenitor program after fertilization. Importantly, during spermatogenesis mechanisms of epigenetic regulation on sex chromosomes are different from autosomes: X-linked somatic/progenitor genes are suppressed by meiotic sex chromosome inactivation without deposition of H3K27me3.ConclusionsOur results suggest that bivalent H3K27me3 and H3K4me2/3 domains are not limited to developmental promoters (which maintain bivalent domains that are silent throughout the reproductive cycle), but also underlie reversible silencing of somatic/progenitor genes during the mitosis-to-meiosis transition in late spermatogenesis.

【 授权许可】

Unknown   
© Sin et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.