The Effect of Early Mosquito Insecticides Exposure on Spraque Dawley Rat Testis: A Histopathological Feature Towards Malignancy?

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Winarni, Tri Indah^1 ; Aziman, Milzam Auzan^1 ; Andar, Anindyo Abshar^1 ; Pawitra, Ika^2

Department of Anatomy, Faculty of Medicine, Diponegoro University, Semarang, Indonesia^1

Department of Patology Anatomy, Faculty of Medicine, Diponegoro University, Semarang, Indonesia^2

关键词: Endocrine disrupting chemicals;    Endocrine disruption;    Endocrine disruptor;    Germ cells;    Histological features;    Leydig cells;    malignancy;    Mosquito repellents;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012023/pdf
DOI  :  10.1088/1755-1315/55/1/012023

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The incidence of health problems associated with endocrine-disruption have increased. Many studies suggesting that endocrine disruptor chemicals (EDC) do contribute to cancer through estrogen-related receptors. Many chemicals have EDCs properties including insecticides. Early life exposure to EDCs can increased the risk of testicular cancer have been reported in the last decade. This study was aimed to determine the effect of insecticides exposure on histopathological tumor cell development of germ and Leydig cell. True experiment research design with posttest only control group design was applied. Sprague Dawley (SD) rat (n = 25) were randomly divided into 5 groups (control group, 25 mg β estradiol 3-benzoate, spiral mosquito coil repellent, 3 ml of liquid mosquito repellent, and 4 ml of liquid mosquito repellent). The exposure were administered for 20 days started at aged 3 days. At the age of 100 days (older adult), testis was stained using Hematoxyllin Eosin (HE) and histological features predicting malignancy were observed. The number of tumor cell development in both testicular germ cells and Leydig cells significantly increased in all treated group compared to those of control and the changes towards malignancy were also observed in all treated group. Exposure to mosquito insecticides causes significant changes in testicular germ and Leydig cell histological features that leads to malignancy.