Microbial production of vitamin B12: a review and future perspectives

Microbial Cell Factories

Review

Jie Kang¹  Dawei Zhang²  Huan Fang³ 

[1] Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China;College of Biotechnology and Food Science, Tianjin University of Commerce, 300134, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China;Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China;Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China;Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China;University of Chinese Academy of Sciences, 100049, Beijing, China;

关键词: Vitamin B;    Biosynthesis;    Metabolic regulation;    Synthetic biology;    Escherichia coli;    Metabolic engineering;   

DOI  :  10.1186/s12934-017-0631-y

 received in 2016-09-18, accepted in 2017-01-20,  发布年份 2017

来源: Springer

【 摘 要 】

Vitamin B12 is an essential vitamin that is widely used in medical and food industries. Vitamin B12 biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. Rational strain engineering is dependent on efficient genetic tools and a detailed knowledge of metabolic pathways, regulation of which can be applied to improve product yield. Recent advances in synthetic biology and metabolic engineering have been used to efficiently construct many microbial chemical factories. Many published reviews have probed the vitamin B12 biosynthetic pathway. To maximize the potential of microbes for vitamin B12 production, new strategies and tools are required. In this review, we provide a comprehensive understanding of advances in the microbial production of vitamin B12, with a particular focus on establishing a heterologous host for the vitamin B12 production, as well as on strategies and tools that have been applied to increase microbial cobalamin production. Several worthy strategies employed for other products are also included.

【 授权许可】

CC BY   
© The Author(s) 2017