Engineered Vesicles for Perchlorate Degradation

Polymer vesicles;Perchlorate treatment;Synthetic biology;Water treatment

Poust, Sean K. ; Zilles ; Julie L.

关键词: Polymer vesicles;    Perchlorate treatment;    Synthetic biology;    Water treatment;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/17064/Poust_Sean.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.