Evaluation of lytic bacteriophages for control of multidrug-resistant Salmonella Typhimurium

Annals of Clinical Microbiology and Antimicrobials

Research

Tian Ding¹  Lae-seung Jung²  Juhee Ahn³ 

[1] Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, 310058, Hangzhou, Zhejiang, China;Department of Medical Biomaterials Engineering, Kangwon National University, 24341, Chuncheon, Gangwon, Republic of Korea;Department of Medical Biomaterials Engineering, Kangwon National University, 24341, Chuncheon, Gangwon, Republic of Korea;Institute of Bioscience and Biotechnology, Kangwon National University, 24341, Chuncheon, Gangwon, Republic of Korea;

关键词: Salmonella;    Bacteriophage;    Antibiotic resistance;    Lytic activity;    Ciprofloxacin;   

DOI  :  10.1186/s12941-017-0237-6

 received in 2017-06-05, accepted in 2017-09-13,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe emergence of antibiotic-resistant bacteria can cause serious clinical and public health problems. This study describes the possibility of using bacteriophages as an alternative agent to control multidrug-resistant Salmonella Typhimurium.MethodsThe potential lytic bacteriophages (P22-B1, P22, PBST10, PBST13, PBST32, and PBST 35) were characterized by morphological property, heat and pH stability, optimum multiplicity of infection (MOI), and lytic activity against S. Typhimurium KCCM 40253, S. Typhimurium ATCC 19585, ciprofloxacin-induced antibiotic-resistant S. Typhimurium ATCC 19585, and S. Typhimurium CCARM 8009.ResultsP22-B1 and P22 belong to Podoviridae family and PBST10, PBST13, PBST32, and PBST 35 show a typical structure with polyhedral head and long tail, belonging to Siphoviridae family. Salmonella bacteriophages were highly stable at the temperatures (< 60 °C) and pHs (5.0–11.0). The reduction rates of host cells were increased at the MOI-dependent manner, showing the highest reduction rate at MOI of 10. The host cells were most effectively reduced by P22, while P22-B1 showed the least lytic activity. The ciprofloxacin-induced antibiotic-resistant S. Typhimurium ATCC 19585, and clinically isolated antibiotic-resistant S. Typhimurium CCARM 8009 were resistant to ciprofloxacin, levofloxacin, norfloxacin, and tetracycline. P22 showed the highest lytic activity against S. Typhimurium KCCM 40253 (> 5 log reduction), followed by S. Typhimurium ATCC 19585 (4 log reduction) and ciprofloxacin-induced antibiotic-resistant S. Typhimurium ATCC 19585 (4 log reduction).ConclusionThe results would provide vital insights into the application of lytic bacteriophages as an alternative therapeutics for the control of multidrug-resistant pathogens.

【 授权许可】

CC BY   
© The Author(s) 2017