Effectiveness of chitosan from crab shell as antibiotic for Escherichia coli

4th International Conference on Biological Sciences and Biotechnology

Boy, E.^1 ; Ummah, Z.A.^1

Faculty of Medicine, Muhammadiyah North Sumatra University, Jl. Gedung Arca, No. 53, Medan, Indonesia^1

关键词: Animal resource;    Antibiotic effects;    Antibiotic resistance;    Chitosan solution;    Effective concentration;    Experimental methods;    Kruskal-Wallis tests;    Natural ingredients;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012010/pdf
DOI  :  10.1088/1755-1315/305/1/012010

来源: IOP

【 摘 要 】

Background: Research in finding a safe, effective and natural ingredient against Escherichia coli continues to be carried out as a result of an increasing antibiotic resistance. Chitosan has been known as a food preservative but the research for its effectivity against Eschericia coli is still inadequate. Indonesia is rich of natural animal resources including crustaceans, like Crab. Objective : How effective are chitosans extracted from crab shells as an antibiotic against Escherichia coli when compared with ceftriaxone. Method : Experimental method with a static group comparison where E. Coli in Mueller Hinton Agar (MHA) on petri dishes was given chitosan intervention with initiating concentrations starting from 4% to 8% and 30mcg of ceftriaxone given by diffusion. The antimicrobial of E.coli was measured with Whatman paper and standard calipers measured in millimeters. Result : Results of normality and homogeneity variance tests with (p > 0,05) were inhomogenous and not distributed normally so it was analyzed with Kruskal Wallis Test continued with a Mann Whitney Test. Conclusion : Chitosan solution extracted from crab shells with concentrations of 4%, 5%, and 6% have an antibiotic effect against the growth of E.coli. The most effective concentration to inhibit E.coli is 4%.