Genetic transformation of mouse cultured cells with the help of high‐velocity mechanical DNA injection

FEBS Letters

Zelenin, Alexander V.¹  Kolesnikov, Viktor A.¹  Titomirov, Alexander V.¹ 

[1] Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Vavilova Street 32, Moscow 117984, USSR

关键词: Transfection;    High-velocity mechanical DNA injection;    Antibiotic resistance;    (NIH 3T3 cell);   

DOI  :  10.1016/0014-5793(89)81163-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of 5–20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization.

【 授权许可】

Unknown