Transcription of Drosophila mobile element gypsy (mdg4) in heat‐shocked cells

FEBS Letters

Ilyin, Yu.V.¹  Lyubomirskaya, N.V.¹  Arkhipova, I.R.¹ 

[1] V.A. Engelhardt Institute of Molecular Biology, Academy of Sciences of Russia, Moscow 117984, Russian Federation

关键词: Retrotransposon gypsy;    Drosophila melanogaster cultured cell;    Transfection;    Heat shock;    Transcription;   

DOI  :  10.1016/0014-5793(93)81079-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Drosophila melanogaster Schneider 2 line cultured cells were subjected to stable transformation by co-transfection with two plasmids, one of which conferred G418 resistance and another which contained the Drosophila retrotransposon, gypsy (mdg4), under the control of the heat-shock protein 70 promoter. Transcription of the introduced constructs, as well as of endogenous gypsy, was examined under the condition of heat shock. Active degradation of pre-existing gypsy transcripts was observed. During recovery, gypsy transcription was restored, but its termination and/or 3'-end processing became aberrant.

【 授权许可】

Unknown