The mystery of the trichothecene 3‐O‐acetyltransferase gene

FEBS Letters

Kimura, Makoto¹  Yoneyama, Katsuyoshi¹  Yamaguchi, Isamu¹  Shingu, Yoshinori¹  Matsumoto, Gentaro¹ 

[1] Microbial Toxicology Laboratory, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako, Saitama 351-0198, Japan

关键词: Trichothecene mycotoxin;    Antibiotic resistance;    Biosynthetic gene cluster;    Acetyltransferase;    Independent evolution;    Fusarium graminearum;    Fgpho5;    phosphate permease gene from F. graminearum;    Fgura7;    UTP-ammonia ligase gene from F. graminearum;    FsTri101;    trichothecene 3-O-acetyltransferase gene from F. sporotrichioides;    RT-PCR;    reverse-transcription PCR;   

DOI  :  10.1016/S0014-5793(98)01061-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The trichothecene 3-O-acetyltransferase gene, Tri101, plays a pivotal role for the well-being of the type B trichothecene producer Fusarium graminearum. We have analyzed the cosmids containing Tri101 and found that this resistance gene is not in the biosynthetic gene cluster reported so far. It was located between the UTP-ammonia ligase gene and the phosphate permease gene which are not related to trichothecene biosynthesis. These two ‘house-keeping’ genes were also linked in Fusarium species that do not produce trichothecenes. The result suggests that the isolated occurrence of Tri101 is attributed to horizontal gene transfer and not to the reciprocal translocation of the chromosome containing the gene cluster. Interestingly, 3-O-acetylation was not always a primary self-defensive strategy for all the t-type trichothecene producers; i.e. the type A trichothecene producer Fusarium sporotrichioides did not acetylate T-2 toxin in vivo although the fungus possessed a functional 3-O-acetyltransferase gene. Thus Tri101 appears to be a defense option which the producers have independently acquired in addition to their original resistance mechanisms.

【 授权许可】

Unknown