The trichothecene biosynthesis gene cluster of Fusarium graminearum F15 contains a limited number of essential pathway genes and expressed non‐essential genes

FEBS Letters

Kimura, Makoto⁵  Yamaguchi, Isamu⁵  Hamamoto, Hiroshi²  Ward, Todd J³  Shibata, Takehiko⁴  Tokai, Takeshi⁵  O’Donnell, Kerry³  Fujimura, Makoto¹ 

[1] Faculty of Life Science, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-1093, Japan;Laboratory for Adaptation and Resistance, Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan;Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA;Cellular and Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-1098, Japan;Laboratory for Remediation Research, Plant Science Center, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

关键词: Biosynthesis gene cluster evolution;    Comparative genomics;    Fungal secondary metabolism;    Plant pathogen;    Trichothecene mycotoxin;    Virulence factor;    3-ADON;    3-acetyldeoxynivalenol;    15-ADON;    15-acetyldeoxynivalenol;    CD;    conserved domain;    DON;    deoxynivalenol;    4-ANIV;    4-acetylnivalenol;    NIV;    nivalenol;    RT-PCR;    reverse transcription-PCR;   

DOI  :  10.1016/S0014-5793(03)00208-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report for the first time the complete structure and sequence of the trichothecene biosynthesis gene cluster (i.e. Tri5-cluster) from Fusarium graminearum F15, a strain that produces 3-acetyldeoxynivalenol (3-ADON). A putative tyrosinase and polysaccharide deacetylase gene flank the Tri5-cluster: the number of pathway genes between them is less than half the total number of steps necessary for 3-ADON biosynthesis. In comparison with partial Tri5-cluster sequences of strains with 15-acetyldeoxynivalenol and 4-acetylnivalenol chemotypes, the Tri5-cluster from strain F15 contains three genes that are apparently unnecessary for the biosynthesis of 3-ADON (i.e. Tri8 and Tri3, which are expressed, and pseudo-Tri13, which is not expressed). In addition, the Tri7 gene was missing from the cluster. Recombinant TRI3 protein showed limited trichothecene C-15 acetylase activity. In contrast, recombinant TRI8 protein displayed no C-3 deacetylase activity, suggesting that the loss or alteration of function contribute directly to the chemotype difference.

【 授权许可】

Unknown