Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates

Malaria Journal

Research

Sung-Ung Moon¹  Khin Lin²  Jin-Soo Lee³  Tong-Soo Kim⁴  Woon-Mok Sohn⁵  Byoung-Kuk Na⁵  Hye-Lim Ju⁵  Jung-Mi Kang⁵  Hyeong-Woo Lee⁶  Jung-Yeon Kim⁷ 

[1] Department of Anatomy, Yonsei University College of Medicine, 120-752, Seoul, Korea;Department of Health, Vector Borne Diseases Control Project, 36 Theinbyu Road, Mandalay, Myanmar;Department of Internal Medicine and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 400-712, Incheon, Korea;Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University School of Medicine, 400-712, Incheon, Korea;Department of Parasitology and Institute of Health Sciences, Gyeongsang National University School of Medicine, 660-751, Jinju, Korea;Department of Pathology, University of Florida, J-566, 1600 SW Archer Road, FL 32610, Gainesville, USA;Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, 122-701, Osong, Korea;

关键词: Plasmodium vivax;    Duffy binding protein;    Myanmar;   

DOI  :  10.1186/1475-2875-11-60

 received in 2011-10-24, accepted in 2012-03-01,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPlasmodium vivax Duffy binding protein (PvDBP) plays an essential role in erythrocyte invasion and a potential asexual blood stage vaccine candidate antigen against P. vivax. The polymorphic nature of PvDBP, particularly amino terminal cysteine-rich region (PvDBPII), represents a major impediment to the successful design of a protective vaccine against vivax malaria. In this study, the genetic polymorphism and natural selection at PvDBPII among Myanmar P. vivax isolates were analysed.MethodsFifty-four P. vivax infected blood samples collected from patients in Myanmar were used. The region flanking PvDBPII was amplified by PCR, cloned into Escherichia coli, and sequenced. The polymorphic characters and natural selection of the region were analysed using the DnaSP and MEGA4 programs.ResultsThirty-two point mutations (28 non-synonymous and four synonymous mutations) were identified in PvDBPII among the Myanmar P. vivax isolates. Sequence analyses revealed that 12 different PvDBPII haplotypes were identified in Myanmar P. vivax isolates and that the region has evolved under positive natural selection. High selective pressure preferentially acted on regions identified as B- and T-cell epitopes of PvDBPII. Recombination may also be played a role in the resulting genetic diversity of PvDBPII.ConclusionsPvDBPII of Myanmar P. vivax isolates displays a high level of genetic polymorphism and is under selective pressure. Myanmar P. vivax isolates share distinct types of PvDBPII alleles that are different from those of other geographical areas. These results will be useful for understanding the nature of the P. vivax population in Myanmar and for development of PvDBPII-based vaccine.

【 授权许可】

Unknown   
© Ju et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.