Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions

BMC Genomics

Research Article

Se Kye Kim¹  Kyoung Hwa Jung¹  Young Gyu Chai² 

[1] Department of Molecular and Life Science, Hanyang University ERICA, 55 Hanyangdaehak-ro, Sangnok-gu, 15588, Ansan, Gyeonggi-do, Republic of Korea;Department of Molecular and Life Science, Hanyang University ERICA, 55 Hanyangdaehak-ro, Sangnok-gu, 15588, Ansan, Gyeonggi-do, Republic of Korea;Department of Bionanotechnology, Hanyang University, 222 Wangsimni-ro, 04763, Seongdong-gu, Seoul, Republic of Korea;

关键词: Bacillus anthracis;    CodY;    Environmental signals;    Starvation;    RNA-sequencing;    Chromatin immunoprecipitation;   

DOI  :  10.1186/s12864-016-3004-8

 received in 2016-05-12, accepted in 2016-08-09,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHost-specific environmental factors induce changes in Bacillus anthracis gene transcription during infection. A global transcription regulator, CodY, plays a pivotal role in regulating central metabolism, biosynthesis, and virulence in B. anthracis. In this study, we utilized RNA-sequencing to assess changes in the transcriptional patterns of CodY-regulated B. anthracis genes in response to three conditions of environmental starvation: iron, CO2, or glucose deprivation. In addition, we performed chromatin immunoprecipitation on newly identified CodY-mediated genes.ResultsEnvironmental deprivation induced transcriptional changes in CodY-regulated genes in both wild-type and codY null strains, and both CodY-specific and environment-specific patterns were observed. In the iron-depleted condition, overexpression of iron homeostasis genes was observed independent of codY deletion; however, transcription of siderophore and amino acid biosynthesis genes was CodY dependent. Although CodY has a significant regulatory role in central metabolism and the carbon overflow pathway, metabolism-associated genes exhibited CodY-independent expression patterns under glucose starvation. Genes that were differentially expressed in response to CO2 availability showed CodY-dependent regulation, though their maximal expression did require a supply of CO2/bicarbonate.ConclusionsWe speculate that CodY regulates the expression of environmental-responsive genes in a hierarchical manner and is likely associated with other transcription regulators that are specific for a particular environmental change.

【 授权许可】

CC BY   
© The Author(s). 2016