In vivo immediate early gene expression induced in intestinal and colonic mucosa by feeding

FEBS Letters

DuBois, Raymond N.¹  Holt, Peter R.² 

[1] Department of Molecular Biology and Genetics, Johns Hopkins Medical Center, Baltimore, MD, USA;Division of Gastroenterology, St. Luke's/Roosevelt Hospital Center and Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10025, USA

关键词: Starvation;    Refeeding;    Gene activation;    Immediate early gene: Intestinal gene regulation;   

DOI  :  10.1016/0014-5793(91)80025-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Since the gut responds rapidly to food intake, the levels of expression of several immediate early genes were measured in mucosa from small and large intestine of rats starved for 3 days or refed. Within 1 h of refeeding, jejunal and ileal c-fos, jun B and zif/268 mRNA and colonil zif/268 dramatically increased. The zif/268 gene in jejunum corresponded in size to the full-length cDNA but. in ileum, an RNA band of about 1.2 kb in size increased greatly after feeding. This represents a physiologic in vivo model for the study of gene regulation associated with intestinal epithelial cellular responses to feeding.

【 授权许可】

Unknown