Isolation and analysis of a multifunctional triterpene synthase KcMS promoter region from mangrove plant kandelia candel

3rd International Conference on Biological Sciences and Biotechnology

Basyuni, M.^1,3 ; Wati, R.^1 ; Sulistiyono, N.^1 ; Sumardi^2 ; Oku, H.^3 ; Baba, S.^4 ; Sagami, H.^5

Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1, Medan, North-Sumatera

20155, Indonesia^1

Faculty of Pharmacy, Tjut Nyak Dhien University, Jl. Rasmi No. 28, Medan

20123, Indonesia^2

Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^3

International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^4

Institute of Multidisciplinary Research for Advanced Material, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai

980-8577, Japan^5

关键词: Abiotic stress;    Cis-acting element;    Environmental signals;    leaf;    Molecular cloning;    Regulatory elements;    Regulatory motifs;    Terpenoids;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012013/pdf
DOI  :  10.1088/1755-1315/130/1/012013

来源: IOP

【 摘 要 】

Molecular cloning of Kandelia candel KcMS gene has previously been cloned and encoded a multifunctional triterpene synthase. In this study, the KcMS gene promoter was cloned through Genome walking, sequenced, and analyzed. A 1,358 bp genomic DNA fragment of KcMS promoter was obtained. PLACE and PlantCARE analysis of the KcMS promoter revealed that there was some regulatory elements in response to environmental signals and involved in the regulation of gene expression. Results showed that four kinds of elements are regulated by hormone binding, namely 2 MeJA-responsiveness elements (CGTCA-motif and TGACG-motif), the ABRE (TACGTG) involved in abscisic acid responsiveness, gibberellin-related GARE-motif (AAACAGA), and the TGA-element (AACGAC) as an auxin-responsive element. Several elements in the KcMS have been shown in other plants to be responsive to abiotic stress. These motifs were MBS (CAACTG), TC-rich repeats, and eight light responsive elements. The KcMS promoter was also involved in the activation of defense genes in plants such as HSE (AAAAAATTC) and four circadian control elements (CAANNNNATC). The presence of multipotential regulatory motifs suggested that KcMS may be involved in regulation of plant tolerance to several types of stresses.