Genome sequence analysis of predicted polyprenol reductase gene from mangrove plant kandelia obovata

3rd International Conference on Biological Sciences and Biotechnology

Basyuni, M.^1 ; Sagami, H.^2 ; Baba, S.^3 ; Oku, H.^4

Department of Forestry, Faculty of Forestry, University of Sumatera Utara, Jl. Tri Dharma Ujung No. 1, Medan, North Sumatera

20155, Indonesia^1

Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai

980-8577, Japan^2

International Society for Mangrove Ecosystems, Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^3

Molecular Biotechnology Group, Tropical Biosphere Research Center, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa

903-0213, Japan^4

关键词: Biosynthetic pathway;    dolichol;    Genome sequence data;    Genome sequences;    leaf;    Partial sequences;    polyisoprenoid;    Sequence identity;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012039/pdf
DOI  :  10.1088/1755-1315/130/1/012039

来源: IOP

【 摘 要 】

It has been previously reported that dolichols but not polyprenols were predominated in mangrove leaves and roots. Therefore, the occurrence of larger amounts of dolichol in leaves of mangrove plants implies that polyprenol reductase is responsible for the conversion of polyprenol to dolichol may be active in mangrove leaves. Here we report the early assessment of probably polyprenol reductase gene from genome sequence of mangrove plant Kandelia obovata. The functional assignment of the gene was based on a homology search of the sequences against the non-redundant (nr) peptide database of NCBI using Blastx. The degree of sequence identity between DNA sequence and known polyprenol reductase was confirmed using the Blastx probability E-value, total score, and identity. The genome sequence data resulted in three partial sequences, termed c23157 (700 bp), c23901 (960 bp), and c24171 (531 bp). The c23157 gene showed the highest similarity (61%) to predicted polyprenol reductase 2- like from Gossypium raimondii with E-value 2e-100. The second gene was c23901 to exhibit high similarity (78%) to the steroid 5-alpha-reductase Det2 from J. curcas with E-value 2e-140. Furthermore, the c24171 gene depicted highest similarity (79%) to the polyprenol reductase 2 isoform X1 from Jatropha curcas with E- value 7e-21.The present study suggested that the c23157, c23901, and c24171, genes may encode predicted polyprenol reductase. The c23157, c23901, c24171 are therefore the new type of predicted polyprenol reductase from K. obovata.