Characterization of the human STAT5A and STAT5B promoters: evidence of a positive and negative mechanism of transcriptional regulation

FEBS Letters

D'Urso, Michele¹  Ursini, Matilde Valeria¹  Sanzari, Emma¹  Monfregola, Jlenia¹  Fimiani, Giorgia¹  Ambrosio, Raffaele¹  Crispi, Stefania¹  De Felice, Nicola¹ 

[1] Institute of Genetics and Biophysics ‘Adriano Buzzati-Traverso’, CNR, Via P. Castellino 111, 80131 Naples, Italy

关键词: STAT5A;    STAT5B;    Epigenetic control;    Transcriptional regulation;    Cis-acting element;    Cross-species comparison;    STAT;    signal transducer and activator of transcription;    GM-CSF;    granulocyte-macrophage colony-stimulating factor;    IL-2;    interleukin 2;    PCR;    polymerase chain reaction;    CNS;    conserved non-coding sequence;    UTR;    untranslated region;   

DOI  :  10.1016/S0014-5793(04)00166-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently published the genomic characterization of the STAT5A and STAT5B paralogous genes that are located head to head in the 17q21 chromosome and share large regions of sequence identity. We here demonstrate by transient in vitro transfection that STAT5A and STAT5B promoters are able to direct comparable levels of transcription. The expression of basal promoters is enhanced after Sp1 up-regulation in HeLa and SL2 cells while DNA methylation associated to the recruitment of MeCP2 methyl CpG binding protein down-regulates STAT5A and B promoters by interfering with Sp1-induced transcription. In addition, cross-species sequence comparison identified a bi-directional negative cis-acting regulatory element located in the STAT5 intergenic region.

【 授权许可】

Unknown