Understanding cellular internalization pathways of silicon nanowires

Journal of Nanobiotechnology

Research

Yimin Huang¹  Kelly McNear¹  Chen Yang² 

[1] Department of Chemistry, Purdue University, 560 Oval Drive, 47907, West Lafayette, IN, USA;Department of Chemistry, Purdue University, 560 Oval Drive, 47907, West Lafayette, IN, USA;Department of Physics and Astronomy, Purdue University, 47907, West Lafayette, IN, USA;

关键词: Cellular interaction;    Silicon nanowires;    Membrane wrapping;   

DOI  :  10.1186/s12951-017-0250-0

 received in 2016-09-08, accepted in 2017-02-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundUnderstanding how cells interact with nanomaterials is important for rational design of nanomaterials for nanomedicine and transforming them for clinical applications. Particularly, the mechanism for one-dimensional (1D) nanomaterials with high aspect ratios still remains unclear.ResultsIn this work, we present amine-functionalized silicon nanowires (SiNW-NH2) entering CHO-β cells via a physical membrane wrapping mechanism. By utilizing optical microscopy, transmission electron microscopy, and confocal fluorescence microscopy, we successfully visualized the key steps of internalization of SiNW-NH2 into cells.ConclusionOur results provide insight into the interaction between 1D nanomaterials and confirm that these materials can be used for understanding membrane mechanics through physical stress exerted on the membrane.

【 授权许可】

CC BY   
© The Author(s) 2017