Triterpenoids from Acacia ataxacantha DC: antimicrobial and antioxidant activities

BMC Complementary and Alternative Medicine

Research Article

Mélanie Bourjot¹  Cathérine Vonthron-Senecheau¹  Ambaliou Sanni²  Latifou Lagnika²  Abdou Madjid O. Amoussa³ 

[1] Laboratoire d’Innovation Thérapeutique, Faculté de Pharmacie, UMR CNRS-Unistra 7200, Illkirch, France;Unité de Biochimie et Biologie Moléculaire, Equipe de Biochimie et Substances Naturelles Bioactives, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 04 BP 0320, Cotonou, Bénin;Unité de Biochimie et Biologie Moléculaire, Equipe de Biochimie et Substances Naturelles Bioactives, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 04 BP 0320, Cotonou, Bénin;Laboratoire d’Innovation Thérapeutique, Faculté de Pharmacie, UMR CNRS-Unistra 7200, Illkirch, France;

关键词: Acacia ataxacantha;    Triterpene;    Betulinic acid-3-trans-caffeate;    Antibacterial;    Antifungal;    Antioxidant;   

DOI  :  10.1186/s12906-016-1266-y

 received in 2016-01-16, accepted in 2016-08-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAcacia ataxacantha is a medicinal specie used extensively in traditional medicine of Benin republic to treat infectious diseases. Our previous study showed interesting antibacterial and antifungal activities against six strains of bacteria and six strains of fungi. The aim of this study was to investigate the antimicrobial and antioxidant activities of compounds isolated from A. ataxacantha.MethodsChromatographic and spectroscopic methods were used to isolate and identify three compounds (1–3) from the bark of A. ataxacantha. Phytochemical investigation of A. ataxacantha (Fabaceae) led to the isolation of three triterpenoids (1–3). The structure of isolated compounds was established by differents spectroscopic methods such as UV, 1H NMR, 13C NMR, 2D NMR and Mass. All isolated compounds were tested for antimicrobial activity using agar disc-diffusion and microdilution methods. The radical scavenging activity of isolated compounds was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method.ResultsPhytochemical investigation led to the isolation and identification of lupeol (1), betulinic acid (2) and betulinic acid-3-trans-caffeate (3). Moderate antimicrobial activity was obtained with compound 3 against methicillin-resitant Staphylococcus aureus, Enterococcus feacalis and Pseudomonas aeruginosa with MIC value of 25 μg/ml and Staphylococcus aureus (MIC of 50 μg/ml). Compounds 3 was more active against Staphylococcus epidermidis and Candida albicans with a MIC value of 12.5 μg/ml in boths cases. Compounds 3 had also interesting antioxidant activity with an IC50 of 3.57 μg/ml compared to quercetin (1.04 μg/ml).ConclusionThe overall results of this study provide evidence that the compound 3, isolated from A. ataxacantha, exhibit antimicrobial activity against Gram-positive and Gram-negative bacteria and yeast, especially against C. albicans.

【 授权许可】

CC BY   
© The Author(s). 2016