Antibacterial activities of triterpenoidal compounds isolated from Calothamnus quadrifidus leaves

BMC Complementary and Alternative Medicine

M. R. Elgindi¹  R. R. Ibrahim¹  D. G. El-Hosari¹  H. A. Ibrahim¹ 

[1] Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, EinHelwan, 11795, Cairo, Egypt;

关键词: Antibacterial;    Calothamnus quadrifidus;    Myrtaceae;    Triterpene;   

DOI  :  10.1186/s12906-019-2512-x

来源: Springer

【 摘 要 】

BackgroundCalothamnus quadrifidus R.Br has many traditional uses and there are few reports about its chemical and biological activities. So our aim is to isolate the triterpenoidal compounds from dichloromethane fraction (DCMF) of Calothamnus quadrifidus R.Br leaves and in addition to evaluate the antibacterial activity of the isolated compounds.MethodsDCMF of C. quadrifidus leaves was subjected to different chromatographic techniques to isolate pure triterpenoidal compounds which were identified using different chemical and spectroscopic techniques. Antibacterial activities of the isolated compounds were evaluated using agar well diffusion method while minimum inhibitory concentration was assessed by microtiter plate assay method.ResultsFive compounds were isolated and they were betulinic acid (1), ursolic acid (2), 3-acetyl-23-hydroxy betulinic acid (3), 2,23-dihydroxy betulinic acid (4) and 2,21,23-trihydroxy betulinic acid (5) were isolated from DCMF of C. quadrifidus leaves for the first time. Compounds 4 and 5 showed strong antibacterial activity against S. typhimurium while compound 4, 5 and 3, 4 exhibits moderate effect against E.coli and S. aureus respectively.ConclusionPure triterpenoidal compounds isolated from C. quadrifidus leaves showed antibacterial activities in different strengths.

【 授权许可】

CC BY