Anti-inflammatory Triterpenes from Euonymus alatus Leaves and Twigs on Lipopolysaccharide-activated RAW264.7 Macrophage Cells

Bulletin of the Korean chemical society

Ji-Yeong Bae¹  Sang Hyun Sung¹  Mi-Jeong Ahn¹  Jung-Rae Rho¹  Young Choong Kim¹  Eun Ju Jeong¹ 

关键词: Euonymus alatus;    Triterpene;    Inflammation;    Nitric oxide;    RAW264.7;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

As a part of ongoing phytochemical research on Euonymus alatus leaves and twigs, we have isolated one new reissantane-type triterpene (1), together with eight known triterpenes, isoarborinol (2), friedelin (3), abruslactone A (4), 3β,22α-dihydroxyolean-12-en-29-oic acid (5), 3α,22α-dihydroxyolean-12-en-29-oic acid (6), 3α,22β-dihydroxyolean-12-en-29-oic acid (7), 22α-hydroxy-3-oxoolean-12-en-29-oic acid (8), demethylregelin (9). The structure of 1 has been elucidated as 24,24-dimethyl-reissant-7(8),25-dien-3α-ol, by extensive 1D and 2D spectroscopic methods including 1H-NMR, 13C-NMR, 1H-1H COSY, HSQC, HMBC and NOESY. Anti-inflammatory activities of the isolated compounds were determined as potential regulating excessive inflammatory responses in RAW264.7 macrophage cells. Particularly, abruslactone A (4) and demethylregelin (9) showed the most potent activity, which effectively reduced the expression of iNOS protein and subsequent nitric oxide production induced by lipopolysaccharide in RAW264.7 cells.

【 授权许可】

Unknown