Diversity of Mycoplasma hominis clinical isolates from Bordeaux, France, as assessed by multiple-locus variable-number tandem repeat analysis

BMC Microbiology

Research Article

Hélène Renaudin¹  Cyril Férandon²  Olivia Peuchant³  Cécile Bébéar³ 

[1] CHU de Bordeaux, Laboratoire de Bactériologie, 33076, Bordeaux, France;Univ. Bordeaux, USC Mycoplasmal and Chlamydial Infections in Humans, 33076, Bordeaux, France;INRA, USC Mycoplasmal and Chlamydial Infections in Humans, 33076, Bordeaux, France;Univ. Bordeaux, USC Mycoplasmal and Chlamydial Infections in Humans, 33076, Bordeaux, France;INRA, USC Mycoplasmal and Chlamydial Infections in Humans, 33076, Bordeaux, France;CHU de Bordeaux, Laboratoire de Bactériologie, 33076, Bordeaux, France;

关键词: Mycoplasma hominis;    MLVA;    Genotyping;   

DOI  :  10.1186/1471-2180-13-120

 received in 2012-11-27, accepted in 2013-05-24,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundMycoplasma hominis is an opportunistic human mycoplasma species that can cause various urogenital infections and, less frequently, extragenital infections. The objective of this work was to study the genetic diversity of this species using a molecular typing method based on multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA).ResultsThe genome content of M. hominis PG21 was analysed for tandem repeats (TRs), and five of the 130 TRs identified were selected for use in an MLVA assay. The method was based on GeneScan analysis of VNTR loci using multiplex PCR with fluorescent dyes and resolution by capillary electrophoresis. This approach was used on a collection of 210 urogenital and extragenital French clinical isolates collected between 1987 and 2009. Forty MLVA types were found. The discriminatory index of our MLVA scheme was 0.924. Using this new typing tool, persistent infection was suggested for six patients and new infection for one patient. Furthermore, mother-to-child transmission was confirmed in the two cases studied. Application of MLVA to a wide range of M. hominis isolates revealed high genotypic diversity and no obvious link between the MLVA type and the isolate year of collection, the patient’s age or sex, the anatomical origin of the isolates or resistance to antibiotics was found.ConclusionsOur MLVA scheme highlights the high genetic heterogeneity of the M. hominis species. It seems too discriminatory to be used for large epidemiological studies but has proven its usefulness for molecular studies at the individual level.

【 授权许可】

Unknown   
© Férandon et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.