The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

BMC Complementary and Alternative Medicine

Research Article

Daxiong Xiang¹  Jieying Luo²  Jingyu He³  Jian Hu⁴  Xiaoke Wen⁴  Zuoqi Xiao⁴  Shuqiong Chen⁴  Wei Zou⁵  Zeneng Cheng⁶ 

[1] Clinic Pharmacy Research Laboratory, the Second Xiangya Hospital of Central South University, 410011, Changsha, China;College of Pharmacy, Hunan University of Chinese Medicine, 410007, Changsha, China;Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, No. 1121 Haibin Road, 511458, Guangzhou, Guangdong, China;Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics and Gynecology Research, Hunan Provincial Maternal and Child Health Care Hospital, No. 53 XiangChun Road, 410008, Changsha, China;Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics and Gynecology Research, Hunan Provincial Maternal and Child Health Care Hospital, No. 53 XiangChun Road, 410008, Changsha, China;School of Pharmaceutical Sciences, Central South University, 410011, Changsha, China;School of Pharmaceutical Sciences, Central South University, 410011, Changsha, China;

关键词: Andrographis paniculata;    Anti-inflammatory;    Pelvic inflammatory disease;    NF-κB;    Rats;   

DOI  :  10.1186/s12906-016-1466-5

 received in 2016-03-30, accepted in 2016-11-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAndrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism.MethodsThirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted.ResultsAPN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway.ConclusionAPN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.

【 授权许可】

CC BY   
© The Author(s). 2016