The inhibitory effect of Andrographis paniculata extract on proliferation of breast cancer cell line

9th Annual Basic Science International Conference 2019

自然科学（总论）

Sholihah, M.M.^1 ; Indarto, D.^1^2 ; Pramana, T.Y.^3

Biomedical Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia^1

Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia^2

Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia^3

关键词: Adenylate kinase;    Andrographis paniculata;    Breast cancer cells;    Cancer cell growth;    Experimental laboratory;    Inhibitory effect;    MCF-7 cells;    Neoandrographolide;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/6/062029/pdf
DOI  :  10.1088/1757-899X/546/6/062029

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Aerobic glycolysis is commonly used as the main energy source for cancer cell growth. Adenylate kinase 2 (AK2) plays an important role in maintenance of ATP production in the mitochondria. Neoandrographolide in Andrographis paniculata potentially inhibit AK2 activity in silico. This study aimed to investigate the inhibitory effect of A. paniculata extract on proliferation of breast cancer cell line. This experimental laboratory used MCF-7 cell line and A. paniculata plants, which were extracted using the maceration method with 70% ethanol. Neoandrographolide concentration in A. paniculata extract was determined using HPLC. A total 1x104 MCF-7 cells were incubated for 24 hours with or without 0.4% (v/v) dimethyl sulfoxide as solvent (SC) or negative control (NC) group. The same amount of MCF-7 cells was treated with A. paniculata extract with 3 different dosages: 222 ppm (AE1), 111 ppm (AE2) and 55.5 ppm (AE3). The inhibition of cell proliferation used the 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide (MTT) assay. Data were analyzed using ANOVA and LSD tests with p value