The Potency of Biologically Synthesized Silver Nanoparticles Using Andrographis Paniculata Extract (S-AgNp) as an Antibacterial Agent

International Conference on Condensed Matters and Advanced Materials 2018

Rupiasih, Ni Nyoman^1 ; Ayu Putu Cyntia Dewi, Gusti^1 ; Wayan Supardi, I.^1 ; Ketut Putra, I.^1

Department of Physics, Faculty of Mathematics and Natural Sciences, Udayana University, Kampus Bukit Jimbaran, Bali, Badung

80361, Indonesia^1

关键词: Andrographis paniculata;    Anti-bacterial activity;    Antibacterial tests;    Distilled water;    Growth inhibition;    Minimum inhibitory concentration;    Negative control;    Pathogenic bacterium;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/515/1/012013/pdf
DOI  :  10.1088/1757-899X/515/1/012013

来源: IOP

【 摘 要 】

This study reported the potency of 'biologically synthesized silver nanoparticles using Andrographis paniculata extract' (S-AgNp) as an antibacterial agent and its minimum inhibitory concentration (MIC). The antibacterial test was conducted to pathogenic bacteria of S. Aureus and E. coli by using a diffusion-well method. The media used were Nutrient Agar (NA) and Nutrient Broth (NB). The tests were carried out in two steps. The first step was determining the potency of S-AgNp as an antibacterial agent. This step used S-AgNp with the concentration of 100%, Andrographis paniculata extract and AgNO3 solution as a positive control, and distilled water as negative control. It was found that S-AgNp were able to inhibit the growth of both bacteria. The second step was finding out the MIC of S-AgNp to both bacteria. In this step, the concentration of S-AgNp tested from 70% to 10% and amoxicillin of 5% was used as the positive control. It was found that the antibacterial activity on both bacteria increased as increasing the concentration of S-AgNp tested. The growth inhibition of S. Aureus was greater than that of E. coli (p