Antibacterial Activity of the Isolation Ethyl Acetate-Soluble Extract Noni Fruit (Morindra citrifolia L.) against Meat Bacterial Decay

International Conference on Advanced Materials for Better Future 2016

Nugraheni, E.R.^1 ; Nurrakhman, M.B.E.^2 ; Munawaroh, H.^3 ; Saputri, L.^3

Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^1

Departement of Biology, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^2

Department of Chemistry, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^3

关键词: Acinetobacter calcoaceticus;    Anti-bacterial activity;    Antibacterial tests;    Bacillus licheniformis;    Chemical preservatives;    Extraction process;    Growth of bacteria;    Klebsiella pneumonia;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/176/1/012007/pdf
DOI  :  10.1088/1757-899X/176/1/012007

来源: IOP

【 摘 要 】

Noni (Morindra citrifolia L.) is native to Indonesia which have medicinal properties. One of them as an antibacterial. This study aims to determine the antibacterial activity of isolates from the ethanol extract noni fruit to bacterial decay meat is Bacillus licheniformis, Klebsiella pneumonia, Bacillus alvei, Acinetobacter calcoaceticus, and Staphylococcus saprophyticus. The extraction process using the maceration method, and then made a partition by centrifugation ethyl acetate. Soluble part partition showed bacterial growth inhibition activity of the strong to very strong. Furthermore, the ethyl acetate soluble partition on preparative thin layer chromatography produced 5 isolates. Isolates obtained antibacterial activity test performed with a concentration of 20% and 30%. The results of antibacterial test against bacteria test isolates, showing isolates A can not inhibit the growth of bacteria, isolates B and C have medium activity and strong, isolates D and E isolates have activity against bacteria that were tested. MIC and MBC test results showed that the isolates B gives an inhibitory effect (bacteriostatic) against all bacteria. Content analysis of compounds by TLC using the reagents cerium (IV) sulfate indicates a phenol group. Isolates B contains a major compound which can be used as an antibacterial candidate in food preservation replace chemical preservatives.