Sesquiterpene lactone containing Mexican Indian medicinal plants and pure sesquiterpene lactones as potent inhibitors of transcription factor NF‐κB

FEBS Letters

Bork, Peter M²  Heinrich, Michael²  Kuhnt, Michaela²  Escher, Claudia¹  Schmitz, M.Lienhard¹ 

[1] Institute of Biochemistry and Molecular Biology, H. Herderstr. 7, Albert-Ludwigs University, 79104 Freiburg, Germany;Institute of Pharmaceutical Biology, Schänzlestr. 1, Albert-Ludwigs University, 79104 Freiburg, Germany

关键词: Transcription factor NF-κB;    Mixe Indian traditional medicine;    Mexico;    Anti-inflammatory;    HET-CAM assay;    Sesquiterpene lactone;    Asteraceae;    AMLE;    Artemisia ludoviciana ssp. mexicana leaf extract;    ASA;    acetylsalicylic acid;    CZLE;    Calea zacatechichi leaf extract;    EMSA;    electrophoretic mobility shift assay;    HET-CAM;    hen egg test-chorioallantoic membrane;    NF-κB;    PDTC;    ammonium pyrrolidinedithiocarbamate;    PMA;    phorbol 12-myristate 13-acetate;    PMLE;    Polymnia maculata leaf extract;    PMSF;    phenylmethylsulfonyl fluoride;    ROIs;    reactive oxygen intermediates;    SDS;    sodium dodecyl sulfate;    SL;    sesquiterpene lactone;   

DOI  :  10.1016/S0014-5793(96)01502-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential inhibitory effect of 54 Mexican Indian medicinal plants on the activation of transcription factor NF-κB was studied. Band-shift experiments identified the ethanolic leaf extracts of Artemisia ludoviciana ssp. mexicana, Calea zacatechichi, and Polymnia maculata (all rich in sesquiterpene lactones) as inhibitors of NF-κB down to a concentration of 25 μg/ml. The sesquiterpene lactones isohelenin and parthenolide prevented NF-κB activation completely as low as 5 μM. Treatment of HeLa cells with leaf extract of A. ludoviciana ssp. mexicana, isohelenin and parthenolide prevented the induction of transcription on the IL-6 promoter. These experiments identify the eudesmanolide and germacranolide type of sesquiterpene lactones as potent non-antioxidant inhibitors of NF-κB. All plants active in the NF-κB assay also showed a delay in the onset of capillary reactions of the allantois membrane in a physiological model for anti-inflammatory activity — the HET-CAM assay.

【 授权许可】

Unknown