Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

BMC Complementary and Alternative Medicine

Research Article

Leila Chekir-Ghedira¹  Nadia Mustapha²  Nouha Nasr²  Imen Mokdad Bzeouich²  Hajer Ben Ghozlen²  Jihed Boubaker³  Kamel Ghedira⁴ 

[1] Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;Higher Institute of Medical Technologies of Tunis, Tunis El Manar University, 2092, Tunis El Manar, Tunisia;Unity of bioactive natural substances and biotechnology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, 5000, Monastir, Tunisia;

关键词: Bioactive compounds;    B16F10;    Apoptosis;    Melanogenesis;    Tyrosinase;    Flavonoids;    Polyphenols;   

DOI  :  10.1186/s12906-015-0743-z

 received in 2014-12-16, accepted in 2015-06-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundHere, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated.MethodsThe Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm.ResultsExtracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells.ConclusionThe tested extracts have a significant biological effect which may be due to their bioactive compounds.

【 授权许可】

CC BY   
© Boubaker et al. 2015