Annona muricata L. extracts decrease melanogenesis in B16F10 mouse melanoma cells

Biomedical Dermatology

Hwa Jun Cha¹  Jung-Eun Ku²  Hyun Kyung Lee⁴  Dahye Joo⁴  Seonghee Jeong⁴  Seong Jin Choi⁴  In-Sook An⁴  Shang Hun Shin⁴  Karam Kim⁴  Kyung-Yun Kim⁵  Sun-Hee Jeong⁶ 

[1] Department of Beauty Art, Faculty of Art, Suwon Women’Department of Cosmetology, Kyung-In Women’Department of Skin Care and Beauty, Osan University, Osan-si, Republic of Korea;Korea Institute of Dermatological Sciences, Cheongju-si, Republic of Korea;URG Inc., Seoul, Republic of Korea;s University, Incheon, Republic of Korea;s University, Suwon-si, Republic of Korea

关键词: Annona muricata;    Melanogenesis;    B16F10;    MITF;    Tyrosinase;   

DOI  :  10.1186/s41702-017-0010-7

学科分类：皮肤病学

来源: Springer

【 摘 要 】

Annona muricata (A. muricata) L. (also known as graviola) contains various antioxidants that have beneficial effects on headaches, hypertension, coughs and asthma. A. muricata L. also has various other physiological effects, such as antispasmodic effects for the treatment of heart conditions, and sedative and nervine effects. In the present study, the effect of A. muricata L. extracts on melanogenesis was investigated and the ensuing inhibitory mechanisms were determined. The inhibitory effects of A. muricata L. extracts on melanogenesis were initially investigated by measuring melanin contents. Subsequently, the ensuing mechanisms were characterized by determining changes in the activity of tyrosinase, which is the rate-limiting step of melanogenesis. Finally, mRNA and protein expression levels of tyrosinase and the melanogenesis-associated transcription factor (MITF) were determined. Decreased melanin contents after treatments with A. muricata L. extracts suggested skin-whitening effects, and these changes were reflected by decreased tyrosinase activities. In addition, tyrosinase mRNA and protein expression levels were regulated by A. muricata L. extracts. MITF is a known key transcription factor of tyrosinase and was transcriptionally regulated by A. muricata L. extracts. Specifically, MITF mRNA expression levels were decreased in the presence of A. muricata L. extracts. Taken together, the melanogenesis-moderating effects of A. muricata L. extracts follow suppression of MITF mRNA expression and subsequent transactivation of tyrosinase. The results of the present study confirm the skin-whitening effects of A. muricata L. and characterize the related molecular mechanisms. These data indicate that A. muricata L. has high potential as an ingredient of skin-whitening cosmetics.

【 授权许可】

CC BY