Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells

BMC Complementary and Alternative Medicine

Research Article

Ajit Kumar Saxena¹  Santosh Guru Kumar¹  Mireille Sylviane Dongmo²  Fabrice Fekam Boyoum³  Jeanne Yonkeu Ngogang⁴  Bruno Moukette Moukette⁴  Constant Anatole Pieme⁵ 

[1] Cancer Pharmacology Division, Indian Institute of Integrative Medicine, 180001, Canal Road, 18001, Jammu, India;Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon;Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, PO Box 812, Yaoundé, Cameroon;Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon;Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon;Cancer Pharmacology Division, Indian Institute of Integrative Medicine, 180001, Canal Road, 18001, Jammu, India;

关键词: Apoptosis;    Membrane mitochondrial potential;    Antiproliferative;    Cell cycle;    A. muricata;   

DOI  :  10.1186/1472-6882-14-516

 received in 2014-06-03, accepted in 2014-12-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAnnona muricata (A. muricata) is widely distributed in Asia, Africa and South America. Different parts of this plant are used to treat several diseases in Cameroon. The aim of this study is to determine the in vitro anti-proliferative effects and apoptotic events of A. muricata extracts on HL-60 cells as well as to quantify its phenols content.MethodsThe cell viability was measured by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay while the changes in morphology of HL-60 cells, membrane mitochondrial potential (MMP) and the cell cycle were used for assessment apoptosis induction.ResultsThe results show that the concentration of phenols, flavonoids and flavonols in the extracts varied depending on the part of the plant. All the extracts tested inhibited the proliferation of HL-60 cells in a concentration dependent manner with IC50 varied from 6–49 μg/mL. The growth inhibition of the cells by extracts was associated with the disruption of MMP, reactive oxygen species (ROS) generation and the G0/G1 cell arrest.ConclusionThese findings suggest that the extracts from A. muricata have strong antiproliferation potential and can induce apoptosis through loss of MMP and G0/G1 phase cell arrest.

【 授权许可】

CC BY   
© Pieme et al.; licensee BioMed Central. 2014