Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells

BMC Complementary and Alternative Medicine

Ajit Kumar Saxena¹  Jeanne Yonkeu Ngogang³  Fabrice Fekam Boyoum²  Bruno Moukette Moukette³  Mireille Sylviane Dongmo⁴  Santosh Guru Kumar¹  Constant Anatole Pieme¹ 

[1] Cancer Pharmacology Division, Indian Institute of Integrative Medicine, 180001, Canal Road, 18001 Jammu, India;Department of Biochemistry, Faculty of Sciences, University of Yaoundé I, PO Box 812, Yaoundé, Cameroon;Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, PO Box 1364, Yaoundé, Cameroon;Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon

关键词: A. muricata;    Cell cycle;    Antiproliferative;    Membrane mitochondrial potential;    Apoptosis;   

Others  :  1089634
DOI  :  10.1186/1472-6882-14-516

 received in 2014-06-03, accepted in 2014-12-09,  发布年份 2014

【 摘 要 】

Background

Annona muricata (A. muricata) is widely distributed in Asia, Africa and South America. Different parts of this plant are used to treat several diseases in Cameroon. The aim of this study is to determine the in vitro anti-proliferative effects and apoptotic events of A. muricata extracts on HL-60 cells as well as to quantify its phenols content.

Methods

The cell viability was measured by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay while the changes in morphology of HL-60 cells, membrane mitochondrial potential (MMP) and the cell cycle were used for assessment apoptosis induction.

Results

The results show that the concentration of phenols, flavonoids and flavonols in the extracts varied depending on the part of the plant. All the extracts tested inhibited the proliferation of HL-60 cells in a concentration dependent manner with IC₅₀ varied from 6–49 μg/mL. The growth inhibition of the cells by extracts was associated with the disruption of MMP, reactive oxygen species (ROS) generation and the G0/G1 cell arrest.

Conclusion

These findings suggest that the extracts from A. muricata have strong antiproliferation potential and can induce apoptosis through loss of MMP and G0/G1 phase cell arrest.

【 授权许可】

   
2014 Pieme et al.; licensee BioMed Central.

【 图 表 】

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