Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment

BMC Biotechnology

Research Article

Zhipeng Li¹  Qingbiao Li¹  Yu Liu¹  Yuanpeng Wang¹  Ning He¹  Peize Liu¹  Zhen Chen² 

[1] Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, 361005, Xiamen, People’s Republic of China;The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, 361005, Xiamen, People’s Republic of China;Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, 361005, Xiamen, People’s Republic of China;The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, 361005, Xiamen, People’s Republic of China;College of Life Sciences, Xinyang Normal University, 464000, Xinyang, Henan, China;

关键词: Bioflocculant;    Alteromonas;    Marine bacterium;    Dye wastewater;   

DOI  :  10.1186/s12896-017-0404-z

 received in 2017-08-01, accepted in 2017-10-31,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater.ResultsA bioflocculant-producing bacterium was isolated from seawater and identified as Alteromonas sp. CGMCC 10612. The optimal carbon and nitrogen sources for the strain were 30 g/L glucose and 1.5 g/L wheat flour. In a 2-L fermenter, the flocculating activity and bioflocculant yield reached maximum values of 2575.4 U/mL and 11.18 g/L, respectively. The bioflocculant was separated and showed good heat and pH stability. The purified bioflocculant was a proteoglycan consisting of 69.61% carbohydrate and 21.56% protein (wt/wt). Infrared spectrometry further indicated the presence of hydroxyl, carboxyl and amino groups preferred for flocculation. The bioflocculant was a nanoparticle polymer with an average mass of 394,000 Da. The purified bioflocculant was able to remove Congo Red, Direct Black and Methylene Blue at efficiencies of 98.5%, 97.9% and 72.3% respectively.ConclusionsThe results of this study indicated that the marine strain Alteromonas sp. is a good candidate for the production of a novel bioflocculant and suggested its potential industrial utility for biotechnological processes.

【 授权许可】

CC BY   
© The Author(s). 2017