Selective apoptotic cell death effects of oral cancer cells treated with destruxin B

BMC Complementary and Alternative Medicine

Research Article

Yew-Min Tzeng¹  Chi-Chiang Yang²  Chung-Hung Tsai³  Shih-Pin Chen⁴  Wei-Yu Tsai⁵  Tsong-Ming Lu⁶  Rosa Huang Liu⁷ 

[1] Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, 168 Jifong East Road, 41349, Wufong District, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 110, Section 1Chien-Kuo North Road, 40201, Taichung, Taiwan;Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;School of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Pathology, Chung Shan Medical University Hospital, Chung Shan Medical University, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;School of Medicine, Chung Shan Medical University, Taichung, Taiwan;Division of Pulmonary Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan;School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 110, Section 1Chien-Kuo North Road, 40201, Taichung, Taiwan;School of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Neurology, Chung Shan Medical University Hospital, Taichung, Taiwan;School of Nutrition, Chung Shan Medical University, Taichung, Taiwan;

关键词: Destruxin;    Cytotoxicity;    Oral cancer;    Apoptosis;   

DOI  :  10.1186/1472-6882-14-207

 received in 2014-03-31, accepted in 2014-06-24,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRecent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, however, data on oral cancer cells especial human are absent.MethodsDestruxin B (DB) was isolated and used to evaluate the selective cytotoxicity with human oral cancer cell lines, GNM (Neck metastasis of gingival carcinoma) and TSCCa (Tongue squamous cell carcinoma) cells, and normal gingival fibroblasts (GF) were also included as controls. Cells were tested with different concentrations of DB for 24, 48, and 72 h by MTT assay. Moreover, the mechanism of cytotoxicity was investigated using caspase-3 Immunofluorescence, annexin V/PI staining, and the expression of caspase-3, Bax, and Bcl-2 by western blotting after treated with different concentrations of DB for 72 h as parameters for apoptosis analyses.ResultsThe results show that DB exhibited significant (p < 0.01) and selective time- and dose-dependent inhibitory effects on GNM and TSCCa cells viability but not on GF cells. The data suggested that DB is capable to induce tumor specific growth inhibition in oral GNM and TSCCa cancer cells via Bax/Bcl-2-mediated intrinsic mitochondrial apoptotic pathway in time- and dose-dependent manners.ConclusionsThis is the first report on the anti-proliferation effect of DB in oral cancer cells. The results reported here may offer further evidences to the development of DB as a potential complementary chemotherapeutic target for oral cancer complications.

【 授权许可】

Unknown   
© Huang Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.