Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress

BMC Complementary and Alternative Medicine

Hsueh-Wei Chang³  Fang-Rong Chang⁷  Hurng-Wern Huang¹  Li-Yeh Chuang²  Jen-Yang Tang⁸  You-Cheng Hseu⁶  Ya-Ching Chan³  Chao-Neng Tseng³  Jin-Ching Lee⁴  Jing-Iong Yang⁵  Chi-Chen Yeh⁷ 

[1] Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;Department of Chemical Engineering, I-Shou University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan;Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung, Taiwan;Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

关键词: Glutathione;    Mitochondrial membrane potential;    ROS;    γ-H2AX;    Apoptosis;    Oral cancer;    Red algae;   

Others  :  1232026
DOI  :  10.1186/1472-6882-12-142

 received in 2012-02-21, accepted in 2012-08-23,  发布年份 2012

【 摘 要 】

Background

Methanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular responses.

Methods

In this study, the methanol extraction product MEGT was used to evaluate the cell growth inhibition in oral cancer cells and its possible mechanism was investigated.

Results

The cell viability of MEGT treated Ca9-22 oral cancer cell line was significantly decreased in a dose–response manner (p < 0.05). The sub-G1 population and annexin V intensity of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.0005 and p < 0.001, respectively). The γH2AX intensities of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.05). The reactive oxygen species (ROS) and glutathione (GSH)-positive intensities of MEGT-treated Ca9-22 oral cancer cells were significantly increased and decreased, respectively, in a dose–response manner (p < 0.05). The DiOC₂(3) intensity for mitochondrial membrane potential (MMP) of MEGT-treated Ca9-22 cancer cells was significantly decreased in a dose–response manner (p < 0.05).

Conclusions

These results indicated that MEGT had apoptosis-based cytotoxicity against oral cancer cells through the DNA damage, ROS induction, and mitochondrial depolarization. Therefore, MEGT derived from the edible algae may have potential therapeutic effects against oral squamous cell carcinoma (OSCC).

【 授权许可】

   
2012 Yeh et al.; licensee BioMed Central Ltd.

【 图 表 】

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