Anti-proliferative effect of methanolic extract of Gracilaria tenuistipitata on oral cancer cells involves apoptosis, DNA damage, and oxidative stress

BMC Complementary and Alternative Medicine

Research Article

Ya-Ching Chan¹  Jin-Ching Lee²  Li-Yeh Chuang³  You-Cheng Hseu⁴  Jen-Yang Tang⁵  Jing-Iong Yang⁶  Chi-Chen Yeh⁷  Fang-Rong Chang⁷  Chao-Neng Tseng⁸  Hsueh-Wei Chang⁹  Hurng-Wern Huang^1,10 

[1] Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Chemical Engineering, I-Shou University, Kaohsiung, Taiwan;Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung, Taiwan;Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung, Taiwan;Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Center of Excellence for Environmental Medicine, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;

关键词: Red algae;    Oral cancer;    Apoptosis;    γ-H2AX;    ROS;    Mitochondrial membrane potential;    Glutathione;   

DOI  :  10.1186/1472-6882-12-142

 received in 2012-02-21, accepted in 2012-08-23,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMethanolic extracts of Gracilaria tenuistipitata (MEGT) were obtained from the edible red algae. Previously, we found that water extract of G. tenuistipitata was able to modulate oxidative stress-induced DNA damage and its related cellular responses.MethodsIn this study, the methanol extraction product MEGT was used to evaluate the cell growth inhibition in oral cancer cells and its possible mechanism was investigated.ResultsThe cell viability of MEGT treated Ca9-22 oral cancer cell line was significantly decreased in a dose–response manner (p < 0.05). The sub-G1 population and annexin V intensity of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.0005 and p < 0.001, respectively). The γH2AX intensities of MEGT-treated Ca9-22 cancer cells were significantly increased in a dose–response manner (p < 0.05). The reactive oxygen species (ROS) and glutathione (GSH)-positive intensities of MEGT-treated Ca9-22 oral cancer cells were significantly increased and decreased, respectively, in a dose–response manner (p < 0.05). The DiOC2(3) intensity for mitochondrial membrane potential (MMP) of MEGT-treated Ca9-22 cancer cells was significantly decreased in a dose–response manner (p < 0.05).ConclusionsThese results indicated that MEGT had apoptosis-based cytotoxicity against oral cancer cells through the DNA damage, ROS induction, and mitochondrial depolarization. Therefore, MEGT derived from the edible algae may have potential therapeutic effects against oral squamous cell carcinoma (OSCC).

【 授权许可】

Unknown   
© Yeh et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.