Concentration effects of grape seed extracts in anti-oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage

BMC Complementary and Alternative Medicine

Research Article

Hsueh-Wei Chang¹  Jen-Yang Tang²  Ming-Feng Hou³  Kun-Tzu Li⁴  Che-Yu Hsieh⁴  Zhi-Wen Yang⁵  Yu-Hsuan Huang⁵  Bing-Hung Chen⁵  Tzu-Fun Fu⁶  Ching-Yu Yen⁷  Hurng-Wern Huang⁸  Sheng-Yang Lee⁹ 

[1] Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan;Research Center of Environmental Medicine, Kaohsiung Medical University, 80708, Kaohsiung, Taiwan;Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan;Department of Oral and Maxillofacial Surgery Chi-Mei Medical Center, Tainan, Taiwan;School of Dentistry, Taipei Medical University, Taipei, Taiwan;Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;School of Dentistry and Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan;

关键词: GSE;    Apoptosis;    Oxidative stress;    DNA damage;    Oral cancer;   

DOI  :  10.1186/s12906-015-0621-8

 received in 2014-11-28, accepted in 2015-02-21,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundGrape seeds extract (GSE) is a famous health food supplement for its antioxidant property. Different concentrations of GSE may have different impacts on cellular oxidative/reduction homeostasis. Antiproliferative effect of GSE has been reported in many cancers but rarely in oral cancer.MethodsThe aim of this study is to examine the antioral cancer effects of different concentrations of GSE in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial function, and DNA damage.ResultsHigh concentrations (50–400 μg/ml) of GSE dose-responsively inhibited proliferation of oral cancer Ca9-22 cells but low concentrations (1–10 μg/ml) of GSE showed a mild effect in a MTS assay. For apoptosis analyses, subG1 population and annexin V intensity in high concentrations of GSE-treated Ca9-22 cells was increased but less so at low concentrations. ROS generation and mitochondrial depolarization increased dose-responsively at high concentrations but showed minor changes at low concentrations of GSE in Ca9-22 cells. Additionally, high concentrations of GSE dose-responsively induced more γH2AX-based DNA damage than low concentrations.ConclusionsDifferential concentrations of GSE may have a differentially antiproliferative function against oral cancer cells via differential apoptosis, oxidative stress and DNA damage.

【 授权许可】

Unknown   
© Yen et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.