Concentration effects of grape seed extracts in anti-oral cancer cells involving differential apoptosis, oxidative stress, and DNA damage

BMC Complementary and Alternative Medicine

Hsueh-Wei Chang⁴  Bing-Hung Chen⁵  Che-Yu Hsieh⁴  Tzu-Fun Fu⁷  Sheng-Yang Lee⁶  Yu-Hsuan Huang⁵  Hurng-Wern Huang¹  Kun-Tzu Li⁴  Jen-Yang Tang⁸  Zhi-Wen Yang⁵  Ming-Feng Hou³  Ching-Yu Yen² 

[1] Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;School of Dentistry, Taipei Medical University, Taipei, Taiwan;Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;School of Dentistry and Wan-Fang Hospital, Taipei Medical University, Taipei, Taiwan;Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

关键词: Oral cancer;    DNA damage;    Oxidative stress;    Apoptosis;    GSE;   

Others  :  1219795
DOI  :  10.1186/s12906-015-0621-8

 received in 2014-11-28, accepted in 2015-02-21,  发布年份 2015

【 摘 要 】

Background

Grape seeds extract (GSE) is a famous health food supplement for its antioxidant property. Different concentrations of GSE may have different impacts on cellular oxidative/reduction homeostasis. Antiproliferative effect of GSE has been reported in many cancers but rarely in oral cancer.

Methods

The aim of this study is to examine the antioral cancer effects of different concentrations of GSE in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial function, and DNA damage.

Results

High concentrations (50–400 μg/ml) of GSE dose-responsively inhibited proliferation of oral cancer Ca9-22 cells but low concentrations (1–10 μg/ml) of GSE showed a mild effect in a MTS assay. For apoptosis analyses, subG1 population and annexin V intensity in high concentrations of GSE-treated Ca9-22 cells was increased but less so at low concentrations. ROS generation and mitochondrial depolarization increased dose-responsively at high concentrations but showed minor changes at low concentrations of GSE in Ca9-22 cells. Additionally, high concentrations of GSE dose-responsively induced more γH2AX-based DNA damage than low concentrations.

Conclusions

Differential concentrations of GSE may have a differentially antiproliferative function against oral cancer cells via differential apoptosis, oxidative stress and DNA damage.

【 授权许可】

   
2015 Yen et al.; licensee BioMed Central.

【 图 表 】

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