Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage

BMC Complementary and Alternative Medicine

Research Article

Chang-Yi Wu¹  Yi-An Chung²  Hui-Ru Wang²  Hsueh-Wei Chang³  Ching-Yu Yen⁴  Ming-Yii Huang⁵  Jen-Yang Tang⁶  Hsun-Shuo Chang⁷  Ih-Sheng Chen⁷  Hurng-Wern Huang⁸ 

[1] Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan;Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;Center for Reseach Resources and Development of Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Oral and Maxillofacial Surgery Chi-Mei Medical Center, Tainan, Taiwan;School of Dentistry, Taipei Medical University, Taipei, Taiwan;Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;Department of Radiation Oncology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan;Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan;Institute of Biomedical Science, National Sun Yat-Sen University, Kaohsiung, Taiwan;

关键词: Cryptocarya concinna;    Cryptocaryone;    Oral cancer;    Apoptosis;    Oxidative stress;    γH2AX;   

DOI  :  10.1186/s12906-016-1073-5

 received in 2015-08-12, accepted in 2016-03-02,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCryptocarya-derived crude extracts and their compounds have been reported to have an antiproliferation effect on several types of cancers but their impact on oral cancer is less well understood.MethodsWe examined the cell proliferation effect and mechanism of C. concinna-derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage.ResultsWe found that CPC dose-responsively reduced cell viability of two types of oral cancer cells (Ca9-22 and CAL 27) in MTS assay. The CPC-induced dose-responsive apoptosis effects on Ca9-22 cells were confirmed by flow cytometry-based sub-G1 accumulation, annexin V staining, and pancaspase analyses. For oral cancer Ca9-22 cells, CPC also induced oxidative stress responses in terms of ROS generation and mitochondrial depolarization. Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells.ConclusionsCPC is a potential ROS-mediated natural product for anti-oral cancer therapy.

【 授权许可】

CC BY   
© Chang et al. 2016