Antimicrobial activities of the methanol extract and compounds from the twigs of Dorstenia mannii (Moraceae)

BMC Complementary and Alternative Medicine

Research Article

Armelle T Mbaveng¹  Victor Kuete²  Veronique P Beng³  Bathelemy Ngameni⁴  Bonaventure T Ngadjui⁵  Jacobus J Marion Meyer⁶  Namrita Lall⁶ 

[1] Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Department of Plant Science, Faculty of Agricultural and Biological Science, 0002, Pretoria, South Africa;Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon;Department of Pharmacy and Traditional Pharmacopoeia, Faculty of Medicine and Biomedical Science, University of Yaoundé I, Yaounde, Cameroon;Department of Pharmacy and Traditional Pharmacopoeia, Faculty of Medicine and Biomedical Science, University of Yaoundé I, Yaounde, Cameroon;Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, Yaounde, Cameroon;Department of Plant Science, Faculty of Agricultural and Biological Science, 0002, Pretoria, South Africa;

关键词: Tuberculosis;    Minimal Inhibitory Concentration;    Crude Extract;    Methanol Extract;    ATCC Strain;   

DOI  :  10.1186/1472-6882-12-83

 received in 2012-03-29, accepted in 2012-06-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundDorstenia mannii (Moraceae) is a medicinal herb used traditionally for the treatment of many diseases. In the present study, the methanol extract of D. mannii and nine of its isolated compounds, namely dorsmanin A (1), B (2), C (3), D (4), E (6), F (7), G (8) dorsmanin I (9) and 6,8-diprenyleriodictyol (5), were tested for their antimicrobial activities against yeast, Mycobacteria and Gram-negative bacteria.MethodsThe microplate alamar blue assay (MABA) and the broth microdilution method were used to determine the minimal inhibitory concentration (MIC) and minimal microbicidal concentration (MMC) of the above extract and compounds on a panel of bacterial species.ResultsThe results of the MIC determinations demonstrated that the methanol extract as well as compounds 3 and 8 were able to prevent the growth of all the fourteen studied microorganisms within the concentration range of 4 to 1024 μg/ml. The lowest MIC value for the methanol extract (64 μg/ml) was obtained on Candida albicans. The lowest value for individual compounds (4 μg/ml) was recorded with compounds 3 on Pseudomonas aeruginosa PA01 and 7 on Eschericia coli ATCC strain. The MIC values recorded with compounds 3 on P. aeruginosa PA01, 6 on C. albicans,7 on P. aeruginosa PA01 and K. pneumoniae ATCC strain and C. albicans, and 8 on P. aeruginosa PA01, PA124, P. stuartii, M. tuberculosis MTCS1 were lower than or equal to those of the reference drugs. MMC values not greater than 1024 μg/ml were recorded on all studied microorganisms with compounds 3 and 8.ConclusionThe overall results of the present investigation provided evidence that the crude extract of D. mannii as well as some of its compounds such compounds 3 and 8 could be a potential source of natural antimicrobial products.

【 授权许可】

CC BY   
© Mbaveng et al.; licensee BioMed Central Ltd. 2012