Gallic acid-grafted chitosan antibacterial hydrogel incorporated with polydopamine-modified hydroxyapatite for enhancing bone healing

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Yanlin Zhu¹  Yuxuan Pang²  Song Zhu²  Ruijuan Niu³  Quan Lin⁴  Lin Guan⁴ 

[1] Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun, China;Department of Prosthodontics, School and Hospital of Stomatology, Jilin University, Changchun, China;Meilong Community Health Service Center, Shanghai, China;State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, China;

关键词: hydrogel;    polydopamine;    gallic acid;    antibacterial;    bone regeneration;   

DOI  :  10.3389/fbioe.2023.1162202

 received in 2023-02-09, accepted in 2023-05-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

An open critical-size bone defect is a major medical problem because of the difficulty in self-healing, leading to an increased risk of bacterial infection owing to wound exposure, resulting in treatment failure. Herein, a composite hydrogel was synthesized by chitosan, gallic acid, and hyaluronic acid, termed “CGH.” Hydroxyapatite was modified with polydopamine (PDA@HAP) and introduced to CGH to obtain a mussel-inspired mineralized hydrogel (CGH/PDA@HAP). The CGH/PDA@HAP hydrogel exhibited excellent mechanical performances, including self-healing and injectable properties. Owing to its three-dimensional porous structure and polydopamine modifications, the cellular affinity of the hydrogel was enhanced. When adding PDA@HAP into CGH, Ca2+ and PO43- could release and then promoted differentiation of BMSCs into osteoblasts. Without any osteogenic agent or stem cells, the area of new bone at the site of defect was enhanced and the newly formed bone had a dense trabecular structure after implanting of the CGH/PDA@HAP hydrogel for 4 and 8 weeks. Moreover, the growth of Staphylococcus aureus and Escherichia coli was effectively inhibited through the grafting of gallic acid onto chitosan. Above, this study provides a reasonable alternative strategy to manage open bone defects.

【 授权许可】

Unknown   
Copyright © 2023 Pang, Guan, Zhu, Niu, Zhu and Lin.